Introduction to super-resolution microscopy.

نویسندگان

  • Masahito Yamanaka
  • Nicholas I Smith
  • Katsumasa Fujita
چکیده

In this review, we introduce the principles of spatial resolution improvement in super-resolution microscopies that were recently developed. These super-resolution techniques utilize the interaction of light and fluorescent probes in order to break the diffraction barrier that limits spatial resolution. The imaging property of each super-resolution technique is also compared with the corresponding conventional one. Typical applications of the super-resolution techniques in biological research are also introduced.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Super-Resolution Microscopy: A Virus’ Eye View of the Cell

It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy. However, novel super-resolution microscopy techniques have cast aside technical limitations to reveal a nanoscale view of virus replication and cell biology. This article provides an introduction to super-resolution imaging...

متن کامل

Easy Ways to High- and Super-Resolution Fluorescence Microscopy: Image Scanning Microscopy (ISM), and Stochastic Optical Fluctuation Imaging (SOFI), and Metal-Induced Energy Transfer (MIET)

Classical fluorescence microscopy is limited in resolution by the wavelength of light (diffraction limit) restricting lateral resolution to ca. 200 nm, and axial resolution to ca. 500 nm (at typical excitation and emission wavelengths around 500 nm). However, recent years have seen a tremendous development in highand super-resolution techniques of fluorescence microscopy, pushing spatial resolu...

متن کامل

Probing Amyloid Aggregation and Morphology In Situ by Multiparameter Imaging and Super-Resolution Fluorescence Microscopy

Chapter Outline Introduction 105 Multi-Parameter Microscopy of Protein Aggregation Kinetics 106 Fusion Protein Labeling of Amyloidogenic Proteins 106 Principle of Multi-Parameter Imaging Techniques 107 Fluorescence Anisotropy Imaging 107 Principle of HomoFRET in the Study of Protein SelfAssembly Reactions 107 HomoFRET Imaging for the Study of α-synuclein Aggregation 108 Fluorescence Lifetime Im...

متن کامل

Super-resolution of Defocus Blurred Images

Super-resolution is a process that combines information from some low-resolution images in order to produce an image with higher resolution. In most of the previous related work, the blurriness that is associated with low resolution images is assumed to be due to the integral effect of the acquisition device’s image sensor. However, in practice there are other sources of blurriness as well, inc...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Microscopy

دوره 63 3  شماره 

صفحات  -

تاریخ انتشار 2014